DOI: https://doi.org/10.55373/mjchem.v26i4.114

Keywords: High-purity PGV-0; pentagamavunon-0 synthesis; purification improvement; recrystallization; rinse solvent

Abstract

Recent advancements in nanotechnology have led to a growing demand for pentagamavunon-0 (PGV-0) synthesis. PGV-0 is a curcumin analogue with various pharmacological effects, and its synthesis has been improved for consistency. However, the purity of PGV-0 remains unclear. This study aims to advance a high-purity PGV-0 synthesis method via purification, recrystallization, and isolation from the rinse solvent. The two-hour synthesis was conducted using vanillin and cyclopentanone. Crude PGV-0 was extracted with a 1:1 mixture of acetic acid and distilled water, followed by rinsing with ethanol and boiling distilled water, and recrystallization with hot ethanol and iced-cooled distilled water. The chemical structure was verified by 1H-NMR spectra, while purity was examined using TLC, melting point and range, and HPLC. After rinsing and recrystallization, we acquired 9.497 g of synthesized PGV-0 with a purity of 99.29 %, a melting point of 204.0 - 205.5 °C, and a melting range of 1.3 ± 0.27 °C. The yield from the rinse solvent was 3.161 g, with a purity of 99.18 %. This study presents a novel method for isolating PGV-0 from the rinse solvent and establishes the foundation for future optimization of high-purity PGV-0 synthesis.