DOI: https://doi.org/10.55373/mjchem.v26i4.76

Keywords: Rourea oligophlebia Merr.; radical scavenging activity; total phenolic content; total flavonoid content; response surface methodology; extraction; Box-Behnken design

Abstract

In the present study, the Box-Behnken design (BBD) of the response surface methodology (RSM) was used to investigate the effects of three factors; extraction temperature (°C), extraction time (min), and ethanol concentration (%), of Rourea oligophlebia Merr. stems on the responses of total phenolic content (TPC), total flavonoid content (TFC), and DPPH radical scavenging activity (DPPH). The optimal conditions from response RSM were observed to be 52% v/v of ethanol concentration, 61°C of extraction temperature, and 140 min of extraction time. The experimental values of TPC, TFC, and DPPH radical scavenging activity were 146.9±0.5 mgGAE/g DW, 18.3±0.2 mgQE/g DW, and 93.2±0.3%, respectively.