Keywords: Persicaria odorata; High Performance Liquid Chromatography (HPLC); method validation; Quercetin; Vietnamese coriander

Abstract

Persicaria odorata is also known as Vietnamese coriander or daun kesum, an herbal plant enriched with chemical constituents and numerous health benefits associated with its consumption such as a potential drug against COVID-19. Determination of the presence of quercetin in Persicaria odorata leaves was optimised by using High-Performance Liquid Chromatography coupled with Ultraviolet (HPLC-UV). In this study, the Persicaria odorata leaves were macerated in methanol prior to analysis with the C18 column as the stationary phase at 368 nm. The optimisation was achieved with a mixture of methanol-water (70:30) with a flow rate of 0.3 mL/minute, and separation was achieved in less than 7 minutes. A linear concentration range for quercetin determination was obtained in the range of 10-200 ppm with a limit of detection (LOD) and limit of quantification (LOQ) at 18 ppm and 56.32 ppm, respectively. The recovery percentage ranged from 77.6% to 88.9%. The method was simple, accurate, and successfully applied to determine quercetin in Persicaria odorata leaves using HPLC.