DOI: https://doi.org/10.55373/mjchem.v26i1.332

Keywords: Benzylaminopurine; Kaempferia parviflora; micropropagation; silver nitrate surface sterilization

Abstract

Continuous supply of Kaempferia parviflora plantlets often disrupted by long rhizome bud dormancy period. Rhizome imports from Thailand are required which inflates the cultivation cost. Hence, this study was conducted to establish an alternative cultivation method through in vitro propagation. Rhizome buds were surface sterilized with 100% Clorox® for 3 min, 95% ethyl alcohol for 30 s, 5% Clorox® for 15 min and 0.10% mercuric chloride (HgCl₂) for 10 min giving the highest disinfection percentage (40%). Then, K. parviflora in vitro multiplication were investigated using combinations of benzylaminopurine (BAP) and silver nitrate (AgNO3). Optimum responses were observed in Murashige and Skoog (MS) media with 8 mg/L BAP and 1 mg/L AgNO3 after 30 days, producing shoot number per explant (4.90±0.57), shoot length per explant (5.07±0.33 cm) and root number per explant (9.70±0.58). The results illustrated explants good responses towards BAP and AgNO3 treatments, highlighting in vitro propagation as an alternative for K. parviflora cultivation.