DOI: https://doi.org/10.55373/mjchem.v25i5.101

Keywords: Ligninolytic; wood rot fungus; indigenous

Abstract

Exploration of the ligninolytic indigenous wood rot fungus from tropical areas such as Indonesia has not been widely carried out. The aims of this study were carried out to the species level identification of KLUM1 and KLUM2 from the Kediri Cocoa Plantation and PnUM from the forest of Pujon Malang at East Java Indonesia through phylogenetic analysis based on the Internal Transcribed Sequence (ITS) sequences and identification of their ligninolytic activity in standard Kirk medium. The isolates KLUM1 and KLUM2 were suspected to be novel strains because KLUM1 only had 99% similarity with Myceliophtora thermophilia and KLUM2 of 97% with Thermothelomyces guttulata, while PnUM was identified as Phialemonium inflatum because it had 100 % similarity. All of the three isolates have the ability to produce LiP and MnP which are more dominant than laccase in standard Kirk medium. Thus, the three isolates have the potential to be explored as a source of ligninase enzymes. The exploration of the ligninase ability of these three isolates in various mediums will be interesting for further research in an effort to produce ligninase that is unique and has high activity.