DOI: https://doi.org/10.55373/mjchem.v25i4.165

Keywords: Coriolopsis aspera; RSM; total polyphenol content (TPC); total flavonoid content (TFC); DPPH radical scavenging activity

Abstract

Ultrasound-assisted extraction (UAE) is a process that accelerates the diffusion of substances in cells to liquids, thus decreasing extraction time. However, there is little research on the effects of sonication on the yunzhi mushroom, Coriolopsis aspera. This study investigated the effects of 4 solvents (acetone, methanol, water and ethanol) on the total polyphenol content (TPC), total flavonoid content (TFC), total triterpenoid content (TTC) and antioxidant capacity in fruiting bodies of C. aspera. Ethanol was used for extraction of the fruiting bodies. The results of the BBD experiment for optimization of this process were: TPC = 7.8832 ± 0.1844 mg GAE/gDW, TFC = 1.3521 ± 0.015 mg QE/gDW, TTC = 2.09 ± 0.01394 mg OAE/gDW, and RSA = 4.5832± 0.0455 (µg acid ascorbic/ml), at an extraction temperature of 40 °C, extraction time of 8.04 hours, and ethanol concentration of 79.6 %. At a solid to liquid ratio of 1:53, the experimental results did not differ much from the predicted results, indicating that these conditions were suitable for the extraction of polyphenols, flavonoids and triterpenoids from C. aspera.