MALAYSIAN JOURNAL OF CHEMISTRY (MJChem)

MJChem is double-blind peer reviewed journal published by the Malaysian Institute of Chemistry (Institut Kimia Malaysia) E-ISSN: 2550-1658

Immobilized Lipase-Catalysed Synthesis of Fructose Oleate Ester in Ionic Liquid [Bmim][TfO] and tert-Butanol Solvent Systems

Mohd Basyaruddin Abdul Rahman
Universiti Putra Malaysia
Mohd Rizal Chumati
Universiti Putra Malaysia
Noor Fazrieyana Hamidon
Universiti Putra Malaysia
Azren Aida Asmawi
Universiti Putra Malaysia
Muhammad Alif Mohammad Latif
Universiti Putra Malaysia
Emilia Abdulmalek
Universiti Putra Malaysia

DOI: https://doi.org/10.55373/mjchem.v25i1.91

Keywords: Fructose oleate ester; surfactant; sugar ester; lipase; ionic liquids

Abstract

Fructose oleate is one of the most sought-after sugar esters, but its production is challenging due to the poor solubility of its substrates. However, 1-butyl-3-methylimidazolium (Bmim)-based ionic liquids can solubilize a wide range of substrates and prevent denaturation of enzymes as their physical and chemical properties can be easily tailored. In this work, the enzymatic synthesis of fructose esters using immobilized lipase was investigated using a series of Bmim-based ionic liquids and tert-butanol as solvents. The reaction conditions were optimized for different esterification parameters, such as fatty acid chain length, enzyme loading, reaction temperature and time. The immobilized lipase was found to be more stable in [Bmim][TfO] and had a high affinity towards the monounsaturated acyl donor, oleic acid. Optimum ester conversion (73%) was achieved under ideal reaction conditions comprising low enzyme loading (5% w/w) and a reaction temperature of 60 °C for 72 hours. The synthesis of fructose oleate in [Bmim][TfO] under these optimized conditions has great potential for use in many applications.

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Published 09 March 2023


Issue Vol 25 No 1 (2023): Malaysian Journal of Chemistry

Section