DOI: https://doi.org/10.55373/mjchem.v24i4.68

Keywords: Orange I, salting-out liquid-liquid extraction, beverage sample

Abstract

In this study, a fast and simple salting-out assisted liquid-liquid extraction (SALLE) followed by UV-Visible spectrophotometric method was developed and applied for the determination of Orange I in beverage. The sample treatment is based on the use of water-miscible solvent as the extractant and phase separation under high-salt conditions. Water-miscible extraction solvent was added to aqueous solution (10 mL) before the addition of (NH4)2SO4. The mixture was vortexed until (NH4)2SO4 dissolved. Then, the mixture was centrifuged. The target analytes in the sample were extracted into the organic phase. The aqueous phase was transferred to a vial for analysis. The absorbance of aqueous phase was measured by UV-Visible spectrophotometric method at the wavelength of 475 nm. The effects of parameters such as type of salt, amount of salt, pH, extraction solvent and contact time were evaluated. Under optimum conditions, a good linearity was obtained in the range of 5 – 20 ppm correlation coefficient of 0.9918. The limit of detection (LOD) and limit of quantification (LOQ) were 0.019 ppm and 0.057 ppm, respectively. The percentage of relative standard deviation (RSD%) of intra-day (n = 3) and inter-day (n = 9) were 2.54 and 8.45, respectively. The efficiency of the developed method was proven in the analysis of Orange I in commercial fruit juice sample with the recovery of 85.19%.