MALAYSIAN JOURNAL OF CHEMISTRY (MJChem)

MJChem is double-blind peer reviewed journal published by the Malaysian Institute of Chemistry (Institut Kimia Malaysia) E-ISSN: 2550-1658

Bioassay-guided Fractionation, LC/MS Analysis and In Vitro Cytotoxic Activity of Eriocaulon cinereum R. Br on T47D Breast Cancer Cells

Arde Toga Nugraha
Universitas Islam Indonesia
Sista Werdyani
Universitas Islam Indonesia
Dian Nida Salsabila
Universitas Islam Indonesia
Arba Pramundita Ramadani
Universitas Islam Indonesia
Rollando
Ma Chung University
Mohamad Nurul Azmi Mohamad Taib
Universiti Sains Malaysia

DOI: https://doi.org/10.55373/mjchem.v24i4.82

Keywords: Eriocaulon cinereum R. Br., Rumput Gong, T47D cells, Cytotoxicity

Abstract

In this study, we examined the cytotoxic activity of Eriocaulon cinereum R.Br. against breast cancer T47D cells and normal Vero cells. The plant material of E. cinereum was extracted with n-hexane followed by a series of extraction with ethyl acetate and finally with methanol. The ethyl acetate extract was then fractionated with dichloromethane and ethyl acetate. The dichloromethane fraction was concentrated, then fractionate using a preparative TLC. The subfractions obtained probably contained terpenoids (SF1), flavonoids (SF2), and steroids (SF3) as the three major compounds based on staining reagent and MS identifications. Later, the subfractions; SF1‒SF3 were subjected to cytotoxic activity on breast cancer T47D cells. The result showed that the IC50 values against T47D cells are 84.8 ± 7.8, 89.7 ± 3.4, and 135 ± 12 μg/mL, respectively. In contrast, IC50 values against Vero cells were > 300 µg/mL for all compounds. Among them, the SF1 subfraction shown the good cytotoxic activity compared to other subfractions and has the potential to be further developed.

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Published 31 December 2022


Issue Vol 24 No 4 (2022): Malaysian Journal of Chemistry

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