DOI: https://doi.org/10.55373/mjchem.v24i4.127

Keywords: Dipterocarpaceae, oligostilbenes, HPLC, vaticaphenol A

Abstract

A plant from the Dipterocarpaceae family, Dipterocarpus semivestitus was studied for its oligostilbene components, as they exhibit a wide range of biological activities. The objective of this study is to determine a fast and effective method for the isolation of the oligostilbenes. Two methanol extracts of plant parts, the stem and leaves were subjected to ultra-high-performance liquid chromatography (UHPLC) for profile analysis and preparative high-performance liquid chromatography (prep HPLC) to isolate the compounds. Vaticaphenol A, a stilbene tetramer was isolated, and its structure was elucidated and characterized by nuclear magnetic resonance (NMR) spectral data analysis. The compound was eluted at 12.7 minutes (tR), by applying a gradient mobile phase of ACN: H2O (15:85 to 95:5 for 14 minutes) at 1 ml/min on an analytical scale. Apart from the flow rate, the chromatographic condition was remained similar for preparative scale. The use of different HPLC techniques is an effective method for separating closely eluted compounds. The ability of the systems to develop a suitable chromatographic condition prior to the actual separation process ensures high purity for the isolated compounds.