Keywords: Apocynaceae, Alstonia spathulata, lupeol acetate, stigmasterol

Abstract

In Malaysia, Alstonia spathulata Bl. is locally known as ‘pulai basong’ which distribute in peat forest. The separation of the chemical components from hexane crude extract (bark) of Alstonia spathulata was carried out using different chromatographic techniques (column chromatography and thin layer chromatography). Two compounds were isolated and identified as lupeol acetate 1 and stigmasterol 2. These compounds have never been reported and isolated from this species. The structures of these compounds were elucidated based on the basis of detailed 1DNMR (1H, 13C and DEPT) and 2D-NMR (COSY, HSQC, HMBC) spectroscopic analysis, involving also comparison with data from the literature.