Malaysian Journal of Chemistry, 2017, Vol. 19(1), 67 – 74

In vitro Cytotoxic Activities of Aporphine Alkaloids 
from the Leaves of Alseodaphne peduncularis
(Wall. ex. Ness) Meissn

Ulil Amna, Mohd Azlan Nafiah* and Kartini Ahmad
Department of Chemistry, Faculty of Science and Mathematics,
Universiti Pendidikan Sultan Idris, 35900 Tanjung Malim, Perak, Malaysia

*Corresponding author: (e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.)

 Received: June 2015; Accepted: April 2017


Four known aporphine alkaloids namely laurotetanine 1, N-methylaurotetanine 2, norboldine 3 and boldine 4 were isolated from the leaves of Alseodaphne peduncularis (Wall. Ex. Ness) Meissn (Lauraceae). Phytochemical studies involved extraction, separation, and purification by using various chromatography methods and structural elucidation by using spectroscopic techniques such as UV, IR and 1D and 2D NMR. These alkaloids were assayed for cytotoxicity against human uteric cervical tumor heLa, human promyelocytic leukemia HL-60 and normal mouse fibroblast NIH/3T3 cell lines by using the MTT assay. Alkaloids 1 and 2 showed cytotoxic activity effect against heLa cell line with CD50 value of 2 and 15 μg/ml, respectively, whereas alkaloids 3 and 4 showed very low activity with CD50 value of 42 and 46 μg/ml, respectively. All four alkaloids did not show cytotoxic activity against HL-60 and NIH/3T3 cell lines.

Key wordsAlseodaphne peduncularis; aporphine alkaloids; in vitro cytotoxic; phytochemical studies


Omar, H., Hashim, N. M., Zajmi, A., Nordin, N., Abdelwahab, S. I., Azizan, A. H. S., Hadi, A. H. A. and Ali, H.M. (2013) Aporphine alkaloids from the leaves of Phoebe grandis (Nees) Mer. (Lauraceae) and their cytotoxic and antibacterial activities, Molecules (Basel, Switzerland), 18(8), 8994–9009. doi:10.3390/molecules18088994

Stevigny, C. and Leclercq, J. Q. (2005), Cytotoxic and antitumor potentialities of Aporphinoid alkaloids, Curr. Med. Chem., 5, 173–182.

Gottlieb, O.R. (1972) Chemosystematic of the Lauraceae, Phytochemistry, 11, 1537–1570.

Sun, R., Jiang, H., Zhang, W., Yang, K., Wang, C., Fan, L., He, Q., Feng, J., Du, S., Deng, Z. and Geng, Z. (2014), Cytotoxicity of Aporphine, Protoberberine and Protopine alkaloids from Dicranostigma leptopodum (Maxim.) Fedde, Evidence-Based Complementary and Alternative Medicine, 1–7. doi:10.1155/2014/580483

Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 65, 55–63.

Sangster, A. W. and Stuart, K. L. (1965), Ultraviolet spectra of alkaloids, Chem. Rev., 65.

Williams, D. H. and Fleming, I. (1989), Spectrocopic Methods in Organic Chemistry, (4th edn., Europe, Mc-Graw-Hill Book, pp. 1–29).

Mukhtar, M. R., Awang, K. and Hadi, A. H. A. (2000), Chemical constituents of Phoebe grandis (Nees). Merr. (Lauraceae), Malaysian Journal of Science, 19, 67–70.

Hussain, S. F., Amin, A. and Shamma, M. (1980), The alkaloids of Machillus duthei, J. Chem. Soc. Pak., 2(4), 157–159.

Johns, S. R., Lamberton, J. A. and Sioumis, A. A. (1967), Laurotetanine and N- methyllaurotetanine from Palmeria fengeriana perk. (Family Monimiaceae), Aust. J. Chem, 20, 1787–1788.

Tajudin, T.-J. S. A., Mat, N., SitiAishah, A. B., Yusran, A. A. M., Alwi, A. and Ali, A. M. (2012), Cytotoxity, antiproliferative effects and apoptosis induction of methanolic extract of Cynometra cauliflora Linn. Whole fruit on human promyelocytic leukemia HL-60 Cells, Evidence-Based Complementary and Alternative Medicine: eCAM, 2012, 127373. doi:10.1155/2012/127373.

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