Astilbin, a main component of Smilax glabra Roxb roots of Vietnam was isolated. The structure of astilbin was elucidated by NMR and mass spectra. The astilbin in extracts was analyzed by HPLC, with the content of 21.8% in ethanol extract and 41.5% in ethyl acetate extract. The antioxidant capacity of extracts was evaluated by methods: 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay, thiobarbituric acid-reactive sepecies (TBARS) assay for inhibition of lipid peroxidation via TBARS formed from Fenton system-injured mouse brain, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay for hepatoprotective effect via H2O2-injured mouse hepatocytes. The results indicated ethyl acetate extract with highest content of astilbin showed the most antioxidant capacity in DPPH (SC50: 24.9 µg/ml), TBARS (IC50 9.45) and MTT (ED50: 25.25 µg/ml).
Key words: Smilax glabra Roxb; astilbin; antioxidant activity; DPPH; MDA; MTT; lipid peroxidation.
Camilo, L.A. and Ana, D. (2013) Evaluating the antioxidant capacity of natural products: A review on chemical and cellular-based assays, Analytica Chimica Acta, 6, 1–10.
Carocho, M. and Ferreira, I.C. (2013) A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives, Food Chem. Toxicol., 51,15–25.
Loi, D.T. (2000) The Medical Plants and Natural Dugs in Viet Nam, Medicine Publishing House, Hà Nọi, Viet Nam, P 498–499.
Xu, S., Shang, M.Y., Liu, G.X., Xu, F., Wang, X., Shou, C.C. and Cai, S.Q. (2013) Chemical constituents from the rhizomes of Smilax glabra and their antimicrobial activity, Molecules, 18, 5265–5287.
Zhang, Q.F., Guo, Y.X., Shangguan, X., Zheng, G. and Wang, W.J. (2012) Antioxidant and anti-proliferative activity of Rhizoma Smilacis Chinae extracts and main constituents, Food Chemistry, 133, 140–145.
Zhang, Qing-Feng, Zhong-Rong Zhang and Hon-Yeung Cheung (2009) Antioxidant activity of Rhizoma Smilacis glabrae extracts and its key constituent-astilbin, Food Chemistry, 115, 297–303.
Zang, Q.F., Zhang, Z.R. and Cheung, H.Y. (2008) Hong Kong Pharmaceutical Journal, 15(2), 65–71.
Lu, Y. and Foo, L.Y. (1999) The polyphenol constituents of grape pomace, Food Chemistry, 65, 1–8.
Minh, P.T.H., Tien, N.Q., Hang, T.T.T. and Đien, P.H. (2010) Study on the chemical composition in root of Smilax glabra Roxb., cultvating in Thai Nguyen Province, Journal of Science — Ha noi National University of Education, 55 (6), 62–70.
Xuan, N.N., Phan, Đ.V. and Thuan, N.D. (2000) Vietnam Journal of Pharmacology-Ministry of Health, 4, 12–16 and 11, 18–21.
Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensm-Wiss. U. -Technol., 28, 25–30.
Kumar, G.P., Navyaa, K., Ramya, E.M., Venkataramana, M., Anand, T. and Anilakumar, K.R. (2013) DNA damage protecting and free radical scavenging properties of Terminalia arjuna bark in PC-12 cells and plasmid DNA, Free Rad. Antioxid., 3, 35–39.
Rikans, L.E. and Hornbrook, K.R. (1997) Mini-review: Lipid peroxidation, antioxidant protection and aging, Biochimica et Biophysica Acta, 1362, 116–127.
Pandey, M.K., Mittra, P.K.P. and Mahesh Wari (2012) The lipid peroxidation product as a marker of oxidative stress in epilepsy, Journal of Clinical and Diagnostic Research, 6 (4), 590–592.
Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay, J. Immunol. Methods,65, 55–63.
Chen, H., Yan, X., Zhu, P. and Lin, J. (2006), Antioxidant activity and hepatoprotective potential of agaro-oligosaccharides in vitro and in vivo, Nutrition Journal, 5, 31.